Construct Design for Precise DNA Insertion in Plants
Muzaffar A, Islam MT, Guss AM, Tuskan GA, Chen JG, Yang X.
2026, Trends in Plant Science, https://doi.org/10.1016/j.tplants.2026.01.005
Abstract
Precise insertion of DNA sequences at targeted locations in plant genomes is pivotal for synthetic biology, genetics, and crop improvement. Construct design plays a critical role in achieving precise insertions, yet practical guidance remains limited. This review provides an in-depth overview of construct design principles and targeted DNA insertion (knock-in) strategies in plants. We assess the strengths, limitations, and construct requirements of current knock-in methods for specific applications, including short, large, and multifragment insertions. Additionally, we explore the potential of adopting advanced nonplant technologies to enhance knock-in efficiency and precision in plants. This review provides a valuable resource for facilitating the effective application of knock-in technologies to genetically improve crops with minimal off-target effects.
Citation
Muzaffar A, Islam MT, Guss AM, Tuskan GA, Chen JG, Yang X. (2026) Construct Design for Precise DNA Insertion in Plants. Trends in Plant Science. DOI:10.1016/j.tplants.2026.01.005