Orebaugh et al. (2026) FEMS Microbiology Reviews

Synthetic Soils for Ecological and Synthetic Biology Applications

Orebaugh J, Carrell AA, York LM, Cregger MA, Del Valle Kessra I

2026, FEMS Microbiology Reviews, https://doi.org/10.1093/femsre/fuag012

Abstract

Soils are heterogeneous and dynamic systems characterized by complex physical, chemical, and biological interactions. Understanding these interactions is critical, as they influence plant productivity, global biogeochemical cycles, and ecosystem resilience. While ecologists have long studied soils in field, greenhouse, and laboratory settings, their complexity and heterogeneity make it challenging to pinpoint key properties driving biological processes and derive mechanistic insights. Advancements in synthetic biology, which seeks to engineer and control biological processes in soils, have increased the demand for standardized and controllable experimental platforms. These platforms, referred to here as ‘synthetic soils’, are systems designed to reproduce selected physicochemical characteristics of natural soils in a simplified and defined format, allowing scientists to systematically change soil physicochemical properties (i.e. texture, mineralogy, pH) to study how biological components (i.e. microbes, plants, soil fauna, etc.) respond to, modify, or interact within these controlled environments. This review explores existing synthetic soils, their advantages, limitations, and applications in ecology and synthetic biology, and discusses potential directions for their future development.

Citation

Orebaugh J, Carrell AA, York LM, Cregger MA, Del Valle Kessra I. (2026) Synthetic Soils for Ecological and Synthetic Biology Applications. FEMS Microbiology Reviews DOI:10.1093/femsre/fuag012