Populus transgenic lines used in PMI research

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PMI has generated ~50 transgenic and CRISPR lines in the P. deltoides (WV94 genotype) or P. tremula x P. alba hybrid (717-1B4 genotype) backgrounds. Listed here are transgenic and CRISPR lines that have already been characterized. Additional lines will be added once their expression has been confirmed and characterization has been completed.
Gene	Background	Expression (Confirmation)	Function	References/Contact
PtLecRLK1 (Potri.T022200)	717-1B4 hybrid (Populus tremula x alba)	Overexpression; constitutive (Yes)	L. bicolor colonization	Yao et al. (2024) In prep W. Muchero/J. Chen - ORNL
PtLecRLK1 (Potri.T022200)	WV94 (Populus deltoides)	Overexpression; root specific (Yes)	L. bicolor colonization	Yao et al. (2024) In prep W. Muchero/J. Chen - ORNL
PtSSP1	717-1B4 hybrid (Populus tremula x alba)	Overexpression; constitutive (Yes)	L. bicolor colonization	Liu et al. (2024) submitted X. Yang/J. Chen - ORNL
PtSSP5	717-1B4 hybrid (Populus tremula x alba)	Overexpression, constitutive (Yes)	L. bicolor colonization	X. Yang/J. Chen - ORNL
PtLecRLK-G (Potri.005G018000; Podel.05G017200	WV94 (Populus deltoides)	CRISPRa; activation (Yes)	Septoria susceptibility	X. Yang/J. Chen - ORNL
PtLecRLK-G (Potri.005G018000; Podel.05G017200)	717-1Bf4 hybrid (Populus tremula x alba)	CRISPRa; activation (No)	Septoria susceptibility	X. Yang/J. Chen - ORNL
Angustifolia_11Q (Potri.014G089400)	717-1B4 hybrid (Populus tremula x alba)	Overexpression, constitutive (No)	Defense and hormonal signaling tradeoff	De et al. (2024) In prep W. Muchero/J. Chen - ORNL
Angustifolia_15Q (Potri.014G089400)	717-1B4 hybrid (Populus tremula x alba)	Overexpression, constitutive (No)	Defense and hormonal signaling tradeoff	De et al. (2024) In prep W. Muchero/J. Chen - ORNL
Aiia (AF196486, Bacillus sp. 240B1)	717-1B4 hybrid (Populus tremula x alba)	Overexpression, constitutive (Yes)	Bacterial quorum sensing	reference in progress J. Chen - ORNL/C. Harwood - UW
PtNPR1 (Potri.006G148100)	717-1B4 hybrid (Populus tremula x alba)	Overexpression, constitutive (Yes)	Defense and hormonal signaling tradeoff	reference in progress C. Veneault-Fourrey - INRAE
PtNPR1 (Potri.006G148100)	717-1B4 hybrid (Populus tremula x alba)	RNAi; constitutive (Yes)	Defense and hormonal signaling tradeoff	reference in progress C. Veneault-Fourrey - INRAE
PtJAZ6 (Potri.003G068900)	717-1B4 hybrid (Populus tremula x alba)	RNAi; constitutive (Yes)	L. bicolor colonization	reference in progress C. Veneault-Fourrey - INRAE
Angustifolia knockout	717-1B4 hybrid (Populus tremula x alba)	CRISPR-knockout out (Yes)	Defense and hormonal signaling tradeoff	De et al. (2024) In prep W. Muchero/J. Chen – ORNL