PMI Populus Resources

Populus common gardens

Mouse over or tap the locations on the map for details

Clatskanie (OR)

Corvallis (OR)

Westport (OR)

Boardman (OR)

Davis (CA)

Blount County (TN)

Populus common gardens used for PMI research

PMI has been using natural variants of P. trichocarpa from the GWAS (genome-wide association studies) panel that were clonally replicated in several common gardens including Clatskanie (OR), Boardman (OR), and Davis (CA). In addition, PMI has been leveraging the resources of ’52-124′ QTL P. trichocarpa x P. deltoides pseudo backcross pedigree at Westport (OR). These gardens were originally established by the BioEnergy Science Center and the Center for Bioenergy Innovation (CBI).

Location	Description	References
Clatskanie, OR	~1,100 P. trichocarpa GWAS natural variants (3 replicates); total ~3,300 trees	Evans et al. 2014; Muchero et al. 2015; Simon et al. 2024;
Westport, OR	~350 P. trichocarpa x P. deltoides pseudo-backcross QTL pedigree (3 replicates); total ~1,300 trees	Evans et al. 2014; Muchero et al. 2015; Simon et al. 2020 Simon et al. 2024;
Corvallis, OR	~1,100 P. trichocarpa GWAS natural variants (3 replicates); total ~3,300 trees	Evans et al. 2014; Muchero et al. 2015; Simon et al. 2024;
Davis, CA	~1,500 P. trichocarpa GWAS natural variants (3 replicates); total ~7,600 trees; with irrigation treatment	Muchero et al. 2015; Simon et al. 2024;
Blount County, TN	8 genotypes of P. trichocarpa; 2 genotypes of P. deltoides	Argiroff et al. 2024
Boardman, OR	~1,100 P. trichocarpa GWAS natural variants; total ~3,300 trees; with irrigation treatment	Kristy et al., 2022

Populus species used for PMI research

PMI has been using several different species of Populus including natural variants of P. trichocarpa from the GWAS panel, parents and progeny of the P. trichocarpa x P. deltoides pseudobackcross population as well as wild-type and transgenic plants in the P. deltoides (WV94 genotype) and P. tremula x P. alba hybrid (717-1B4 genotype) backgrounds.

Species/background	Genotype	Description	References
P. tremula x P. alba hybrid cross	717-1B4	Most commonly used genotype for transformation	Phytozome - HAP1; Phytozome - HAP2
P. trichocarpa	BESC-24, BESC-375	Multi-platform characterization of intraspecies variation	Evans et al. 2014; Muchero et al. 2015; Simon et al. 2024;
P. trichocarpa	93-968	'Grandparent' for '52-124' QTL pedigree	Muchero et al. 2015;
P. deltoides	ILL-101	'Grandparent' for '52-124' QTL pedigree	Muchero et al. 2015;
P. trichocarpa x P. deltoides hybrid cross	52-225	F1 hybrid (93-868 x ILL-101); Parent for '52-124' QTL pedigree	Muchero et al. 2015; Induri et al. 2012
P. deltoides	D-124	Parent for '52-124' QTL pedigree	Muchero et al. 2015;
P. deltoides	WV94	Second most commonly used genotype for transformation (BESC/CBI); reference for P. deltoides	Phytozome WV94
P. Trichocarpa	Nisqually-1	Reference for P. Trichocarpa	Tuskan et al., 2006
P. trichocarpa	BESC-819	Superior performance in tissue culture	Evans et al. 2014; Muchero et al. 2015; Simon et al. 2024;

Population size of different Populus species used for PMI research

PMI has been using natural variants of P. trichocarpa from the GWAS panel with ~1,500 individual genotypes that are clonally replicated in several common gardens. PMI has also been using the parents and progeny of the P. trichocarpa x P. deltoides pseudobackcross population with ~350 individual genotypes. In addition, PMI has generated ~50 transgenic and CRISPR lines in the P. deltoides (WV94 genotype) or P. tremula x P. alba hybrid (717-1B4 genotype) backgrounds. Furthermore, PMI has access to the natural variants of P. deltoides from the GWAS panel with ~500 individual genotypes, as well as ~10 Populus representative species collected from diverse geographical locations and ecological systems.

Poplar Collections	Species/Background	Description	References
QTL pedigree	P. trichocarpa x P. deltoides hybrid	~350; progeny from 52-225 x D-124	Muchero et al. 2015; Induri et al. 2012
Natural variants	P. deltoides	~500	Fahrenkrog et al. 2017
Representative species	New Populus species	~10	Poplar Innovation Inc. collection
Natural variants	P. trichocarpa	~1,500; Planted in common gardens	Evans et al. 2014; Muchero et al. 2015; Simon et al. 2024;