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PMI Populus Resources

Populus common gardens

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Us Full Map Aspect Ratio 3 2

Clatskanie (OR)

Corvallis (OR)

Westport (OR)

Boardman (OR)

Davis (CA)

Blount County (TN)

Populus common gardens used for PMI research

PMI has been using natural variants of P. trichocarpa from the GWAS (genome-wide association studies) panel that were clonally replicated in several common gardens including Clatskanie (OR), Boardman (OR), and Davis (CA). In addition, PMI has been leveraging the resources of ’52-124′ QTL P. trichocarpa x  P. deltoides pseudo backcross pedigree at Westport (OR). These gardens were originally established by the BioEnergy Science Center and the Center for Bioenergy Innovation (CBI).

LocationDescriptionReferences
Clatskanie, OR~1,100 P. trichocarpa GWAS natural variants (3 replicates); total ~3,300 treesEvans et al. 2014;
Muchero et al. 2015;
Simon et al. 2024;
Westport, OR~350 P. trichocarpa x P. deltoides pseudo-backcross QTL pedigree (3 replicates); total ~1,300 treesEvans et al. 2014;
Muchero et al. 2015;
Simon et al. 2020
Simon et al. 2024;
Corvallis, OR~1,100 P. trichocarpa GWAS natural variants (3 replicates); total ~3,300 treesEvans et al. 2014;
Muchero et al. 2015;
Simon et al. 2024;
Davis, CA~1,500 P. trichocarpa GWAS natural variants (3 replicates); total ~7,600 trees; with irrigation treatmentMuchero et al. 2015;
Simon et al. 2024;
Blount County, TN8 genotypes of P. trichocarpa; 2 genotypes of P. deltoidesArgiroff et al. 2024
Boardman, OR~1,100 P. trichocarpa GWAS natural variants; total ~3,300 trees; with irrigation treatmentKristy et al., 2022

Populus species used for PMI research

PMI has been using several different species of Populus including natural variants of P. trichocarpa from the GWAS panel, parents and progeny of the P. trichocarpa x P. deltoides pseudobackcross population as well as wild-type and transgenic plants in the P. deltoides (WV94 genotype) and P. tremula x P. alba hybrid (717-1B4 genotype) backgrounds.

Species/backgroundGenotypeDescriptionReferences
P. tremula x P. alba hybrid cross717-1B4Most commonly used genotype for transformationPhytozome - HAP1;
Phytozome - HAP2 
P. trichocarpaBESC-24, BESC-375Multi-platform characterization of intraspecies variationEvans et al. 2014;
Muchero et al. 2015;
Simon et al. 2024;
P. trichocarpa93-968'Grandparent' for '52-124' QTL pedigreeMuchero et al. 2015;
P. deltoidesILL-101'Grandparent' for '52-124' QTL pedigreeMuchero et al. 2015;
P. trichocarpa x P. deltoides hybrid cross52-225F1 hybrid (93-868 x ILL-101); Parent for '52-124' QTL pedigreeMuchero et al. 2015;
Induri et al. 2012
P. deltoidesD-124Parent for '52-124' QTL pedigreeMuchero et al. 2015;
P. deltoidesWV94Second most commonly used genotype for transformation (BESC/CBI); reference for P. deltoidesPhytozome WV94
P. TrichocarpaNisqually-1Reference for P. TrichocarpaTuskan et al., 2006
P. trichocarpaBESC-819Superior performance in tissue cultureEvans et al. 2014;
Muchero et al. 2015;
Simon et al. 2024;

 

Population size of different Populus species used for PMI research

PMI has been using natural variants of P. trichocarpa from the GWAS panel with ~1,500 individual genotypes that are clonally replicated in several common gardens. PMI has also been using the parents and progeny of the P. trichocarpa x P. deltoides pseudobackcross population with ~350 individual genotypes. In addition, PMI has generated ~50 transgenic and CRISPR lines in the P. deltoides (WV94 genotype) or P. tremula x P. alba hybrid (717-1B4 genotype) backgrounds. Furthermore, PMI has access to the natural variants of P. deltoides from the GWAS panel with ~500 individual genotypes, as well as ~10 Populus representative species collected from diverse geographical locations and ecological systems.

Poplar CollectionsSpecies/BackgroundDescriptionReferences
QTL pedigreeP. trichocarpa x P. deltoides hybrid~350; progeny from 52-225 x D-124Muchero et al. 2015;
Induri et al. 2012
Natural variantsP. deltoides~500Fahrenkrog et al. 2017
Representative speciesNew Populus species~10Poplar Innovation Inc. collection
Natural variantsP. trichocarpa~1,500; Planted in common gardensEvans et al. 2014;
Muchero et al. 2015;
Simon et al. 2024;