PMI Populus Resources

This table lists the Populus resources created by the PMI SFA.  Please check back as the table is updated periodically

 

Populus common gardens used for PMI research

PMI has been using natural variants of P. trichocarpa from the GWAS (genome-wide association studies) panel that are clonally replicated in several common gardens including Clatskanie (OR) (~1,100 individual genotypes with 3 replicates), Corvallis (OR) (~1,100 individual genotypes with 3 replicates), and Davis (CA) (~1,500 individual genotypes with 3 replicates). PMI has also been using the parents and progeny of the P. trichocarpa x P. deltoides psudobackcross population with ~350 individual genotypes that are clonally replicated (3 replicates) at the Westport (OR) common garden.
LocationDescription
Clatskanie, OR~1,100 P. trichocarpa GWAS natural variants; total ~3,300 trees
Westport, OR~350 P. trichocarpa x P. deltoides pseudo-backcross QTL pedigree; total ~1,300 trees
Corvallis, OR~1,100 P. trichocarpa GWAS natural variants; total ~3,300 trees
Davis, CA~1,500 P. trichocarpa GWAS natural variants; total ~7,600 trees; with irrigation treatment

 

For more details please see the PMI Populus Common Garden Interactive Map 

 

Populus species used for PMI research

PMI has been using several different species of Populus including natural variants of P. trichocarpa from the GWAS (genome-wide association studies) panel, parents and progeny of the P. trichocarpa x P. deltoides psudobackcross population as well as wild-type and transgenic plants in the P. deltoides (WV94 genotype) and P. tremula x P. alba hybrid (717-1B4 genotype) backgrounds.
Species/backgroundGenotypeDescription
P. tremula x P. alba hybrid cross717-1B4Most commonly used genotype for transformation
P. deltoidesWV94Second most commonly used genotype for transformation (BESC/CBI)
P. trichocarpaBESC-819Superior performance in tissue culture
P. trichocarpaBESC-24, BESC-375Multi-platform characterization of intraspecies variation
P. trichocarpa93-968'Grandparent' for QTL pedigree
P. deltoidesILL-101'Grandparent' for QTL pedigree
P. trichocarpa x P. deltoides hybrid cross52-225F1 hybrid (93-868 x ILL-101); Parent for QTL pedigree
P. deltoidesD-124Parent for QTL pedigree

 

Population size of different Populus species used for PMI research

PMI has been using natural variants of P. trichocarpa from the GWAS (genome-wide association studies) panel with ~1,500 individual genotypes that are clonally replicated in several common gardens. PMI has also been using the parents and progeny of the P. trichocarpa x P. deltoides psudobackcross population with ~350 individual genotypes. In addition, PMI has generated ~50 transgenic and CRISPR lines in the P. deltoides (WV94 genotype) or P. tremula x P. alba hybrid (717-1B4 genotype) backgrounds. Furthermore, PMI has access to the natural variants of P. deltoides from the GWAS panel with ~500 individual genotypes, as well as ~10 Populus representative species collected from diverse geographical locations and ecological systems.
Poplar CollectionsSpecies/BackgroundDescription
Transgenic and CRISPR lines717-1B4 or WV94~50; Populus Transgenics
QTL pedigreeP. trichocarpa x P. deltoides hybrid~350; progeny from 52-225 x D-124
Natural variantsP. trichocarpa~1,500; Planted in common gardens
Natural variantsP. deltoides~500
Representative speciesNew Populus species~10; Poplar Innovation Inc. collection