Lectin-functionalized poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) surface scaffolds for high avidity microbial capture
Ryan R. Hansen, Juan Pablo Hinestrosa, Katherine R. Shubert, Jennifer L. Morrell-Falvey, Dale A. Pelletier, Jamie M. Messman, S. Michael Kilbey, Bradley S. Lokitz, and Scott T. Retterer
2013 September 4, Biomacromolecules 14(10): 3742-3748
Microbial exopolysaccharides (EPS) play a critical and dynamic role in shaping the interactions between microbial community members and their local environment. The capture of targeted microbes using surface immobilized lectins that recognize specific extracellular oligosaccharide moieties offers a nondestructive method for functional characterization of EPS content. In this report, we evaluate the use of the block copolymer, poly(glycidyl methacrylate)-block-4,4-dimethyl-2-vinylazlactone (PGMA-b-PVDMA), as a surface scaffold for lectin-specific microbial capture. Three-dimensional polymer films were patterned on silicon substrates to provide discrete, covalent coupling sites for Triticum vulgare and Lens culinaris lectins. This material increased the number of Pseudomonas fluorescens microbes captured by up to 43% compared to control scaffolds that did not contain the copolymer. These results demonstrate that PGMA-b-PVDMA scaffolds provide a platform for improved microbe capture and screening of EPS content by combining high avidity lectin surfaces with three-dimensional surface topography.
Hanse, R. R., Hinestrosa J. P., Shubert, K. R., Morrell-Falvey, J. L., Pelletier, D. A., Messman, J. M., Kilbey, S. M. II., Bradley S., Lokitz, B. S., and Retterer, S. T. (2013). Lectin-Functionalized Poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) Surface Scaffolds for High Avidity Microbial Capture. Biomacromolecules 2013 14 (10), 3742-3748 DOI: 10.1021/bm4011358