Populus Resources

Populus Species and Genotypes

PMI has been using several different species of Populus including natural variants of P. trichocarpa from the GWAS panel, parents and progeny of the P. trichocarpa x P. deltoides pseudobackcross population, as well as wild-type and transgenic plants in the P. deltoides (WV94 genotype) and P. tremula x P. alba hybrid (717-1B4 genotype) backgrounds.

Species/Cross	Genotype	Description	References
P. tremula x P. alba	717-1B4	Most commonly used genotype for transformation	Phytozome HAP1 Phytozome HAP2
P. deltoides	WV94	Second most commonly used genotype for transformation (BESC/CBI); reference for P. deltoides	Phytozome WV94
P. trichocarpa	BESC-24, BESC-375	Multi-platform characterization of intraspecies variation	Evans et al., 2014 Muchero et al., 2015 Simon et al., 2024
P. trichocarpa	93-968	'Grandparent' for '52-124' QTL pedigree	Muchero et al., 2015
P. deltoides	ILL-101	'Grandparent' for '52-124' QTL pedigree	Muchero et al., 2015
P. trichocarpa x P. deltoides	52-225	F1 hybrid (93-868 x ILL-101); Parent for '52-124' QTL pedigree	Muchero et al., 2015 Induri et al., 2012
P. deltoides	D-124	Parent for '52-124' QTL pedigree	Muchero et al., 2015
P. trichocarpa	Nisqually-1	Reference for P. Trichocarpa	Tuskan et al., 2006
P. trichocarpa	BESC-819	Superior performance in tissue culture	Evans et al., 2014 Muchero et al., 2015 Simon et al., 2024

Populus Collections

PMI has been using ~1,500 individual genotypes of natural variants of P. trichocarpa from the GWAS panel that are clonally replicated in several common gardens. PMI has also been using ~350 individual genotypes from the parents and progeny of the P. trichocarpa x P. deltoides pseudobackcross population. In addition, PMI has generated ~50 transgenic and CRISPR lines in the P. deltoides (WV94 genotype) or P. tremula x P. alba hybrid (717-1B4 genotype) backgrounds. PMI has access to ~500 individual genotypes of natural variants of P. deltoides from the GWAS panel , as well as ~10 Populus representative species collected from diverse geographical locations and ecological systems.

Collection	Species/Cross	Description	References
QTL pedigree	P. trichocarpa x P. deltoides	~350; progeny from 52-225 x D-124	Muchero et al., 2015 Induri et al., 2012
representative species	new Populus species	~10	Poplar Innovation Inc.
natural variants	P. deltoides	~500	Fahrenkrog et al., 2017
natural variants	P. trichocarpa	~1,500; planted in common gardens	Evans et al., 2014 Muchero et al., 2015 Simon et al., 2024

Populus Common Gardens

PMI has been using natural variants of P. trichocarpa from the genome-wide association studies (GWAS) panel that were clonally replicated in several common gardens including Clatskanie, Boardman, and Corvallis, Oregon, and Davis, California. In addition, PMI has been leveraging the resources of ’52-124′ QTL P. trichocarpa x  P. deltoides pseudo backcross pedigree at Westport, Oregon. More recently, PMI maintains natural variants of P. deltoides in two common gardens located in Prosser, Washington, and Knoxville, Tennessee.

 

Location	Description	References
Clatskanie, OR	~1,100 P. trichocarpa GWAS natural variants (3 replicates); total ~3,300 trees	Evans et al., 2014 Simon et al., 2024
Westport, OR	~350 P. trichocarpa x P. deltoides pseudo-backcross QTL pedigree (3 replicates); total ~1,300 trees	Evans et al., 2014 Muchero et al., 2015 Simon et al., 2024 Simon et al., 2020
Corvallis, OR	~1,350 P. trichocarpa GWAS natural variants (3 replicates); total ~4,000 trees	Evans et al., 2014 Simon et al., 2024
Davis, CA	~1,500 P. trichocarpa GWAS natural variants (3 replicates); total ~7,600 trees; irrigation treatment	Simon et al., 2024 Klein et al., 2025
Blount County, TN	8 genotypes of P. trichocarpa; 2 genotypes of P. deltoides	Argiroff et al., 2024
Boardman, OR	~1,100 P. trichocarpa GWAS natural variants; total ~3,300 trees; irrigation treatment	Kristy et al., 2022
Prosser, WA	~550 P. trichocarpa GWAS natural variants (3 replicates); ~350 P. deltoides GWAS natural variants (3 replicates); total ~2,800 trees	Evans et al., 2014 Fahrenkrog et al., 2017 Simon et al., 2024
Knoxville, TN	~350 P. deltoides GWAS natural variants (3 replicates); total ~1,100 trees	Fahrenkrog et al., 2017