Abraham, P. E. et al., 2020. Proteomics

Molecular remodeling in Populus PdKOR RNAi roots profiled using LC-MS/MS proteomics

Paul E. Abraham, Anna Matthiadis, Robert L. Hettich, and Udaya C. Kalluri 
26 August 2020, Proteomics, doi: 10.1002/pmic.202000067

Abstract

Plant endo‐β‐1,4‐glucanases belonging to the Glycoside Hydrolase Family 9 have functional roles in cell wall biosynthesis and remodeling via endohydrolysis of (1→4)‐β‐D‐glucosidic linkages. Modification of cell wall chemistry via RNAi‐mediated downregulation of Populus deltoides KOR1 (PdKOR), a endo‐β‐1,4‐glucanase gene, in Populus deltoides has been shown to have functional consequences for the composition of secondary metabolome and the ability of modified roots to interact with beneficial microbes. The molecular remodeling that underlies the observed differences at metabolic, physiological, and morphological levels in roots is not well understood. Here we used a LC‐MS/MS‐based proteome profiling approach to survey the molecular remodeling in root tissues of PdKOR and control plants. A total of 14316 peptides were identified and these mapped to 7139 P. deltoides proteins. Based on 90% sequence identity, the measured protein accessions represent 1187 functional protein groups. Analysis of GO categories and specific individual proteins showed differential expression of proteins relevant to plant‐microbe interactions, cell wall chemistry, and metabolism. The new proteome dataset serves as a useful resource for deriving new hypotheses and empirical testing pertaining to functional roles of proteins and pathways in differential priming of plant roots to interactions with microbes.

Citation

Abraham, P.E., Matthiadis, A., Hettich, R.L. and Kalluri, U.C. (2020), Molecular remodeling in Populus PdKOR RNAi roots profiled using LC‐MS/MS proteomics. Proteomics. Accepted Author Manuscript. doi:10.1002/pmic.202000067

Outside Links

https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.202000067?af=R