Levy, A. et al., 2017. Nature Genetics

Genomic features of bacterial adaptation to plants

Asaf Levy, Isai Salas Gonzalez, Maximilliam Mittelviefhaus, Scott Clingenpeel, Sur Herrera Paredes, Jiamin Miao, Kunru Wang, Giulia Devescovi, Kyra Stillman, Freddy Monteiro, Bryan Rangel Alvarez, Derek S. Lundberg, Tse-Yuan Lu, Sarah Lebeis, Zhao Jin, Meredith McDonald, Andrew P. Klein, Meghan E. Feltcher, Tijana Glavina del Rio, Sarah R. Gran, Sharon Doty, Ruth Ley, Bingyu Zhao, Vittorio Venturi, Dale A. Pelletier, Julia A. Vorholt, Susannah G. Tringe, Tanja Woyke, and Jeffery L. Dangl.
18 December 2017.  Nature Genetics 50: 138-150 (2018); DOI: 10.1038/s41588-017-0012-9

Abstract

Plants intimately associate with diverse bacteria. Plant-associated bacteria have ostensibly evolved genes that enable them to adapt to plant environments. However, the identities of such genes are mostly unknown, and their functions are poorly characterized. We sequenced 484 genomes of bacterial isolates from roots of Brassicaceae, poplar, and maize. We then compared 3,837 bacterial genomes to identify thousands of plant-associated gene clusters. Genomes of plant-associated bacteria encode more carbohydrate metabolism functions and fewer mobile elements than related non-plant-associated genomes do. We experimentally validated candidates from two sets of plant-associated genes: one involved in plant colonization, and the other serving in microbe–microbe competition between plant-associated bacteria. We also identified 64 plant-associated protein domains that potentially mimic plant domains; some are shared with plant-associated fungi and oomycetes. This work expands the genome-based understanding of plant–microbe interactions and provides potential leads for efficient and sustainable agriculture through microbiome engineering.

Citation

Levy, A., I. Salas Gonzalez, M. Mittelviefhaus, S. Clingenpeel, S. Herrera Paredes, J. Miao, K. Wang, G. Devescovi, K. Stillman, F. Monteiro, B. Rangel Alvarez, D. S. Lundberg, T.-Y. Lu, S. Lebeis, Z. Jin, M. McDonald, A. P. Klein, M. E. Feltcher, T. G. Rio, S. R. Grant, S. L. Doty, R. E. Ley, B. Zhao, V. Venturi, D. A. Pelletier, J. A. Vorholt, S. G. Tringe, T. Woyke and J. L. Dangl (2018). “Genomic features of bacterial adaptation to plants.” Nature Genetics 50(1): 138-150.

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https://www.nature.com/articles/s41588-017-0012-9