Guo, J. et al., 2012. PLoS ONE

Highly Efficient Isolation of Populus Mesophyll Protoplasts and Its Application in Transient Expression Assays

Jianjun Guo, Jennifer L. Morrell-Falvey, Jessy L. Labbé, Wellington Muchero, Udaya C. Kalluri, Gerald A. Tuskan, and Jin-Gui Chen
2012 September 13, PLoS ONE 7(9): e44908.
Download Publication: Guo-journal.pone.0044908.pdf

Abstract

Background: Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.

Methodology/Principal Findings: We optimized a Populus leaf mesophyll protoplast isolation protocol and established a Populus protoplast transient expression system. We demonstrated that Populus protoplasts are able to respond to hormonal stimuli and that a series of organelle markers are correctly localized in the Populus protoplasts. Furthermore, we showed that the Populus protoplast transient expression system is suitable for studying protein-protein interaction, gene activation, and cellular signaling events.

Conclusions/Significance: This study established a method for efficient isolation of protoplasts from Populus leaf and demonstrated the efficacy of using Populus protoplast transient expression assays as an in vivo system to characterize genes and pathways.

Citation

Guo J, Morrell-Falvey JL, Labbé JL, Muchero W, Kalluri UC, et al. (2012) Highly Efficient Isolation of Populus Mesophyll Protoplasts and Its Application in Transient Expression Assays. PLoS ONE 7(9): e44908. doi:10.1371/journal.pone.0044908

Publication Highlight