Arabidopsis C-terminal binding protein ANGUSTIFOLIA modulates transcriptional co-regulation of MYB46 and WRKY33
Meng Xie, Jin Zhang, Tao Yao, Anthony C. Bryan, Yunqiao Pu, Jessy Labbé, Dale A. Pelletier, Nancy Engle, Jennifer L. Morrell-Falvey, Jeremy Schmutz, Arthur J. Ragauskas, Timothy J. Tschaplinski, Feng Chen, Gerald A. Tuskan, Wellington Muchero and Jin-Gui Chen
24-July-2020; New Phytologist 22(5): 1627-1639; doi: https://doi.org/10.1111/nph.16826
- The apparent antagonism between Salicylic Acid (SA) and Jasmonic acid (JA)/Ethylene (ET) signaling resulting in tradeoffs between defense against (hemi)biotrophic and necrotrophic pathogens has been widely described across multiple plant species. However, the underlying mechanism remains to be fully established.
- The molecular and cellular function of ANGUSTIFOLIA (AN) were characterized, and its roles in regulating pathogenic response were studied in Arabidopsis.
- We demonstrated that AN, a plant homolog of mammalian C‐TERMINAL BINDING PROTEIN (CtBP), antagonistically regulates plant resistance to the hemibiotrophic pathogen Pseudomonas syringae and the necrotrophic pathogen Botrytis cinerea . Consistent with the phenotypic observations, transcription of genes involved in SA and JA/ET pathways was antagonistically regulated by AN. By interacting with another nuclear protein TYROSYL‐DNA PHOSPHODIESTERASE1 (TDP1), AN imposes transcriptional repression on MYB46 , encoding a transcriptional activator of PHENYLALANINE AMMONIA‐LYASE (PAL ) genes which are required for SA biosynthesis, while releasing the TDP1‐imposed transcriptional repression on WRKY33 , a master regulator of the JA/ET signaling pathway.
- These findings demonstrate that the transcriptional co‐regulation of MYB46 and WRKY33 by AN mediates the coordination of SA and JA/ET pathways to optimize defenses against (hemi)biotrophic and necrotrophic pathogens.
Xie, M. et al. Arabidopsis C-terminal Binding Protein ANGUSTIFOLIA modulates transcriptional co-regulation of MYB46 and WRKY33. New Phytol. (2020). doi:10.1111/nph.16826